Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Cytotoxicity of berberine and d-limonene, alone and in combination, on the growth of MGC803 cells. MGC803 cells were treated with berberine (C, D) and d-limonene (E, F), alone and in combination (G, H), at 1: 4, for 24 (C, E, G) and 48 h (D, F, H), with cisplatin (A for 24 h, B for 48 h) as a positive control. The viability of cells was qualified by MTT assay, reported as fraction affected (fraction of cells affected by treatment), and data represent the mean±SD from three replicate wells. P<.01, compared with the control group.

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Positive Control, MTT Assay, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Synergistic anticancer effect of the combination of berberine and d-limonene in MGC803 cells. The combination index (CI) (A, B) and dose-reduction index (DRI) (C, D) of the combination of berberine and d-limonene were calculated as described. The data points below CI values of 1, denoted by horizontal dotted lines in (A) and (B), are indicative of synergistic effects.

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques:

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Cell-Cycle Distribution Induced by Berberine and d -Limonene Alone and in Combination in MGC803 Cells

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Induction apoptosis of berberine and d-limonene, alone and in combination, in MGC803 cells. Morphological changes were shown by acridine orange after drug treatment for 48 h: (A) control, (B) 20 μM cisplatin, (C) 20 μM berberine, (D) 80 μM d-limonene, and (E) 20 μM berberine+80 μM d-limonene. The apoptosis induced by berberine and d-limonene at 24, 36, and 48 h was determined by flow cytometry (F). Data are mean±SD values. P<.05, asignificantly different compared with the control group; bsignificantly different compared with berberine group; csignificantly different compared with d-limonene group. The bar is 25 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Control, Flow Cytometry

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Induction of intracellular reactive oxygen species (ROS) generation in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. Monitoring of the ROS production with DCF (A) in MGC803 cells; fluorescence intensity (FI) was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Fluorescence, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Loss of mitochondrial membrane potential (ΔΨm) in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. Detection of ΔΨm changes with Rhodamine 123 (A) in MGC803 cells; FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Membrane, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: The changes of Bcl-2 protein expression in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. The expression of Bcl-2 in MGC803 cells was detected by the FITC-conjugated secondary antibody (A), and FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Expressing, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: The changes in caspase-3 protein expression in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. The expression of caspase-3 (A) in MGC803 cells was detected by the FITC-conjugated secondary antibody, and FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Expressing, Confocal Microscopy, Control

Journal: Cancer gene therapy

Article Title: Midkine and cyclooxygenase-2 promoters are promising for adenoviral vector gene delivery of pancreatic carcinoma.

doi: 10.1038/sj.cgt.7700403

Figure Lengend Snippet: Figure 3. GCV sensitivity of pancreatic carcinoma cell lines transduced with AdCOX-2M Tk, AdCOX-2L Tk, AdCMV Tk, and AdCMV GFP. Established hu- man pancreatic carcinoma cell lines BxPC-3 (A), Capan-1 (B), Hs 766-T (C), MIA PaCa-2 (D), and the human gastric cancer cell line KATO III (E) were infected with AdCMVGFP (normal line), AdCOX-2L Tk (dotted line), AdCOX-2M Tk (striped line), and AdCMV Tk (bold line) at an MOI of 500 for 5 hours. GCV was applied at the concentration ranging from 0 to 10 mM and 5 days later, the percentage of surviving cells was calcu- lated from the number of surviving cells without GCV ( =100%) and the number of surviving cells at various concentra- tions of GCV. The number of surviving cells was determined by an MTT assay. The results are the mean of triplicate assays. Each point represents the mean and the standard deviation of the three determinations.

Article Snippet: KATO III cells (gastric carcinoma) and LS174T cells (colon carcinoma) were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Transduction, Infection, Concentration Assay, MTT Assay, Standard Deviation